Genomic Comparison of cag Pathogenicity Island (PAI)-Positive and -Negative Helicobacter pylori Strains: Identification of Novel Markers for cag PAI-Positive Strains
نویسندگان
چکیده
منابع مشابه
Genomic Comparison of cag pathogenicity island (PAI)-positive and -negative Helicobacter pylori strains: identification of novel markers for cag PAI-positive strains.
In an analysis of Helicobacter pylori genomic DNA by macroarray methodology, genomic DNA from a panel of cag pathogenicity island (PAI)-negative H. pylori clinical isolates failed to hybridize with 27 genes located outside the cag PAI in a cag PAI-positive reference strain. PCR analyses confirmed that HP0217 (encoding a lipopolysaccharide biosynthetic protein) and HP1079 (encoding a protein of ...
متن کاملConserved Transcriptional Unit Organization of the Cag Pathogenicity Island among Helicobacter pylori Strains
The Helicobacter pyloricag pathogenicity island (cag PAI) encodes a type IV secretion system that is more commonly found in strains isolated from patients with gastroduodenal disease than from those with asymptomatic gastritis. Genome-wide organization of the transcriptional units in H. pylori strain 26695 was recently established using RNA sequence analysis (Sharma et al., 2010). Here we used ...
متن کاملInfection by Cag A Positive Strains of Helicobacter pylori is Associated with Autoimmune Thyroid Disease in Iranian Patients
Background: Infectious agents have been suspected as a triggering factor for development of autoimmune thyroid disease (ATD). Some reports from Western countries have suggested association between Helicobacter pylori (HP) infection and ATD. Objective: To investigate the association of ATD with Cag A seropositivity in a population with high rate and early age of onset of HP infection. Methods: I...
متن کاملStructure of cag pathogenicity island in Japanese Helicobacter pylori isolates.
BACKGROUND cag pathogenicity island (PAI) is reported to be a major virulence factor of Helicobacter pylori. AIM To characterise cagA and the cag PAI in Japanese H pylori strains. METHODS H pylori isolates from Japanese patients were evaluated for CagA by immunoblot, for cagA transcription by northern blot, and for cagA and 13 other cag PAI genes by Southern blot. cagA negative strains from...
متن کاملCorrelation between cag pathogenicity island composition and Helicobacter pylori-associated gastroduodenal disease.
Helicobacter pylori infection is associated with a variety of outcomes ranging from seemingly asymptomatic coexistence to peptic ulcer disease and gastric cancer. The cag pathogenicity island (PAI) contains genes associated with a more aggressive phenotype and has been suggested to be a determinant of severe disease outcome. The cagA gene has served as a marker for the cag PAI. However, the pre...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 2005
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.73.6.3794-3798.2005